Liraglutide Protects Nucleus Pulposus Cells Against High-Glucose Induced Apoptosis by Activating PI3K/Akt/ mTOR/Caspase-3 and PI3K/Akt/GSK3β/Caspase-3 Signaling Pathways

Liraglutide Protects Nucleus Pulposus Cells Against High-Glucose Induced Apoptosis by Activating PI3K/Akt/ mTOR/Caspase-3 and PI3K/Akt/GSK3β/Caspase-3 Signaling Pathways

Diabetes mellitus (DM) is reportedly a major threat issue for intervertebral disc degeneration (IDD). Incretin system and notably glucagon-like peptide 1 (GLP-1) due to its glucose-lowering results has turn out to be an vital goal in therapeutic methods of sort 2 diabetes (T2D). Liraglutide is a GLP-1 receptor (GLP-1R) agonist with glucoregulatory and insulinotropic features in addition to regulatory features on cell proliferation, differentiation, and apoptosis. However, little is understood on the roles and signaling pathways of apoptosis defending results of liraglutide in IDD. This examine aimed to research the potential protecting results of liraglutide towards excessive glucose-induced apoptosis of nucleus pulposus cells (NPCs) and the attainable concerned signaling pathways.

The human NPCs have been incubated with 100 nM liraglutide alone or together with LY294002 (PI3K inhibitor), rapamycin (mTOR inhibitor), and SB216763 (GSK3β inhibitor) in a excessive glucose tradition for 48 h. The 4 teams have been assessed additional for apoptosis and genes expressions. The apoptotic impact was evaluated by circulation cytometry and additional confirmed by cell loss of life detection enzyme-linked immunoassay plus (ELISAPLUS). The gene and protein expression ranges have been assessed by quantitative real-time polymerase chain response (qRT-PCR) and Western blotting methods. The outcomes have been comparatively assessed between the 4 teams.

The outcomes confirmed the presence of GLP-1R within the NPCs indicating that liraglutide inhibited the excessive glucose-induced apoptosis, which was blocked by silencing GLP-1R with siRNA. Moreover, liraglutide stimulated the phosphorylation of Akt, mTOR and GSK3β. Treatment with LY294002 considerably elevated the apoptosis of NPCs and diminished the degrees of their downstream substrates (p-AKT, p-mTOR, and p-GSK3β). Further assessments revealed that activation of mTOR and GSK3β was virtually utterly inhibited by rapamycin and SB216763, respectively, which considerably elevated the caspase-Three ranges.

Caspase-3-related apoptosis prevents pathological regeneration in a dwelling liver donor rat mannequin

The principal aim of this examine was to find out the connection of cleaved-caspase-3 (C3)-related apoptosis and hepatic proliferation, through the liver repopulation in a dwelling liver donor rat mannequin. Thirty-three animals have been randomized into eleven teams and evaluated on postoperative from 3 ​h till 384 ​h after 30%-partial hepatectomy (30%-PHx). Liver sections (5 ​μm) have been processed by hematoxylin-eosin, and immunostaining for C3, accompanied by hepatic perform check. C3 content material and the hepatic lobule enlargement have been analyzed by optical density, adopted by cell counting.
Transient variations of alanine transferase (ALT), aspartate aminotransferase (AST) and lactate dehydrogenase (LDH) have been discovered. Significant enhance within the C3 ranges, and cell nuclei quantity, have been detected at 12 ​h and 48 ​h after 30%-PHx, evidencing a correlation of p ​= ​-0.3679. In the 30%-PHx rat mannequin, C3-related apoptosis prevents proliferative pathological circumstances through the hepatic lobule re-modeling.
Cisplatin-induced damage of renal proximal tubular cells outcomes principally from elevated apoptosis through mitochondrial injury, and is mitigated by acceptable enhancement of autophagy. Peroxisome proliferator-activated receptor-delta (PPAR-δ) reportedly protects towards not solely mitochondrial damages but additionally enhances autophagy. Thus, PPAR-δ might shield towards cisplatin-induced kidney damage.
Liraglutide Protects Nucleus Pulposus Cells Against High-Glucose Induced Apoptosis by Activating PI3K/Akt/ mTOR/Caspase-3 and PI3K/Akt/GSK3β/Caspase-3 Signaling Pathways

Cytotoxicity and Toxicological Studies of Artocarpus altilis Extracts, Inducing Apoptosis and Cell Cycle Arrest through CASPASE-3 and CASPASE-8 Pathways Against Human Breast MCF-7 Cells

 

In latest biomedical analysis, the realm of most cancers and infectious illnesses has a number one place within the utilization of medicinal vegetation as a supply of drug discovery. Malaysia has a range and numerous underutilized fruits which might be wealthy in phenolic compounds. Artoarpus altilis take into account an underutilized fruit that’s wealthy in phenolic compounds. Methanol extracts of A. altilis have been beforehand discovered to include a excessive content material of antioxidant phytochemicals.

The goal of the examine was to judge the cytotoxicity and toxicological impact of methanol fruit extracts towards MCF-7 cells. To decide the least focus which may kill or suppress the expansion of the most cancers cells was in a concentration-dependent method strategy. The variation within the cytotoxic exercise among the many extracts was indicated by figuring out the IC50 of every extract towards cells at 72 h. The IC50 of the samples was measured utilizing a trypan blue exclusion assay.

The methanol extract of the pulp half confirmed the least inhibition focus of 15.40±0.91 μg/mL on MCF-7 cells. In the examine, the molecular mechanism of methanol extracts-induced apoptosis and cell cycle arrested in human most cancers cells have been investigated in a time-dependent-manners strategy by utilizing circulation cytometry. The handled cells have been stained with nexin to detect early and late apoptosis and with propidium iodide (PI) for cell cycle arrest related to the DNA fragmentation, numerous cell arrests occurred at G1/S, S, and G2/M phases.

Annexin V-EGFP Apoptosis Kit

K2006-100 100 assays
EUR 516

Annexin V-EGFP Apoptosis Kit

K2006-25 25 assays
EUR 238

Annexin V-EGFP Apoptosis Kit

K104-100
EUR 501

Annexin V-EGFP Apoptosis Kit

K104-25
EUR 207

Annexin V-FITC Apoptosis Kit

K2003-400 400 assays
EUR 1114

Annexin V-Cy3 Apoptosis Kit

K2004-400 400 assays
EUR 1114

Annexin V-Cy5 Apoptosis Kit

K2005-400 400 assays
EUR 1114

Annexin V-Biotin Apoptosis Kit

K2009-400 400 assays
EUR 1114

Annexin V-FITC Apoptosis Kit

K101-400
EUR 1023

Annexin V-Cy3 Apoptosis Kit

K102-400
EUR 958

Annexin V-Cy5 Apoptosis Kit

K103-400
EUR 1023

Annexin V-Biotin Apoptosis Kit

K109-400
EUR 958

Annexin V EGFP Apoptosis Detection Kit

55R-1268 25 assays
EUR 277
Description: Annexin V Apoptosis Kit for detection of apoptosis in the research laboratory

Annexin V-FITC Apoptosis Kit Plus

K2057-400 400 assays
EUR 1059

Annexin V-Cy3 Apoptosis Kit Plus

K2058-400 400 assays
EUR 1059

Annexin V-PE Apoptosis Kit Plus

K2059-400 400 assays
EUR 1059

Annexin V-PE Apoptosis Detection Kit

K2200-400 400 assays
EUR 1114

Annexin V-PE Apoptosis Detection Kit

K128-400
EUR 958

Annexin V-FITC Apoptosis Kit Plus

K201-400
EUR 947

Annexin V-Cy3 Apoptosis Kit Plus

K202-400
EUR 947

Annexin V-PE Apoptosis Kit Plus

K203-400
EUR 947

Annexin V-PE-Cy5 Apoptosis Detection Kit

K2201-400 400 assays
EUR 1253

Annexin V-PE-Cy5 Apoptosis Detection Kit

K129-400
EUR 1039

Annexin V-EGFP / PI Cell Apoptosis Detection Kit

20-abx098247
  • EUR 244.00
  • EUR 314.00
  • 25 rxns
  • 50 rxns
  • Shipped within 5-10 working days.

Annexin V-EGFP Reagent

1004-1000
EUR 1132

Annexin V-EGFP Reagent

1004-200
EUR 403

Annexin V-FITC Apoptosis Kit

K2003-25 25 assays
EUR 238

Annexin V-Cy3 Apoptosis Kit

K2004-25 25 assays
EUR 238

Annexin V-Cy5 Apoptosis Kit

K2005-25 25 assays
EUR 238

Annexin V-Biotin Apoptosis Kit

K2009-25 25 assays
EUR 238

Annexin V-FITC Apoptosis Kit

K101-100
EUR 512

Annexin V-FITC Apoptosis Kit

K101-25
EUR 224

Annexin V-Cy3 Apoptosis Kit

K102-100
EUR 501

Annexin V-Cy3 Apoptosis Kit

K102-25
EUR 207

Annexin V-Cy5 Apoptosis Kit

K103-100
EUR 512

Annexin V-Cy5 Apoptosis Kit

K103-25
EUR 218

Annexin V-Biotin Apoptosis Kit

K109-100
EUR 468

Annexin V-Biotin Apoptosis Kit

K109-25
EUR 207

Annexin V, R-phycoerythrin conjugate (100 assays)

9-29045
  • EUR 149.00
  • EUR 312.00
  • 100uL
  • 500uL
Description: Minimum order quantity: 1 unit of 500uL

Annexin V, Allophycocyanin crosslinked conjugate (100 assays)

9-29057
  • EUR 149.00
  • EUR 312.00
  • 100uL
  • 500uL
Description: Minimum order quantity: 1 unit of 500uL

Annexin V Apoptosis Detection Kit (FITC)

abx290002-100test 100 test
EUR 537
  • Shipped within 3-5 working days.

Annexin V Apoptosis Detection Kit (PE)

abx290003-100test 100 test
EUR 578
  • Shipped within 2-3 weeks.

Annexin V Apoptosis Detection Kit (FITC)

abx290014-200test 200 test
EUR 481
  • Shipped within 3-5 working days.

Annexin V Apoptosis Detection Kit (PE)

abx290015-200test 200 test
EUR 537
  • Shipped within 3-5 working days.

Annexin V Apoptosis Detection Kit (Biotin)

abx290017-200test 200 test
EUR 620
  • Shipped within 2-3 weeks.

Annexin V-FITC Apoptosis Kit Plus

K2057-100 100 assays
EUR 516

Annexin V-FITC Apoptosis Kit Plus

K2057-25 25 assays
EUR 251

Annexin V-Cy3 Apoptosis Kit Plus

K2058-100 100 assays
EUR 516

Annexin V-Cy3 Apoptosis Kit Plus

K2058-25 25 assays
EUR 251

Annexin V-PE Apoptosis Kit Plus

K2059-100 100 assays
EUR 516

Annexin V-PE Apoptosis Kit Plus

K2059-25 25 assays
EUR 251

Annexin V-PE Apoptosis Detection Kit

K2200-100 100 assays
EUR 516

Annexin V-PE Apoptosis Detection Kit

K2200-25 25 assays
EUR 238

Annexin V-PE Apoptosis Detection Kit

K128-100
EUR 501

Annexin V-PE Apoptosis Detection Kit

K128-25
EUR 207

Annexin V-FITC Apoptosis Kit Plus

K201-100
EUR 501

Annexin V-FITC Apoptosis Kit Plus

K201-25
EUR 240

Annexin V-Cy3 Apoptosis Kit Plus

K202-100
EUR 490

Annexin V-Cy3 Apoptosis Kit Plus

K202-25
EUR 240

Annexin V-PE Apoptosis Kit Plus

K203-100
EUR 485

Annexin V-PE Apoptosis Kit Plus

K203-25
EUR 234

Annexin V Apoptosis Detection Kit (FITC)

55R-1265 25 assays
EUR 277
Description: Annexin V Apoptosis Kit for detection of apoptosis in the research laboratory

Annexin V Apoptosis Detection Kit (CY3)

55R-1266 25 assays
EUR 277
Description: Annexin V Apoptosis Kit for detection of apoptosis in the research laboratory

Annexin V Apoptosis Detection Kit (CY5)

55R-1267 25 assays
EUR 277
Description: Annexin V Apoptosis Kit for detection of apoptosis in the research laboratory

Annexin V Apoptosis Detection Kit (biotin)

55R-1271 25 assays
EUR 277
Description: Annexin V Apoptosis Kit for detection of apoptosis in the research laboratory

Annexin V Apoptosis Detection Kit (PE)

55R-1288 25 assays
EUR 277
Description: Annexin V Apoptosis Kit for detection of apoptosis in the research laboratory

Annexin V (FITC) / PI Apoptosis Detection Kit

abx090601-100tests 100 tests
EUR 460
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Annexin V / PI Apoptosis Detection Kit (FITC)

abx290007-100test 100 test
EUR 453
  • Shipped within 3-5 working days.

Annexin V / 7ADD Apoptosis Detection Kit (FITC)

abx290008-100test 100 test
EUR 453
  • Shipped within 3-5 working days.

Annexin V / 7AAD Apoptosis Detection Kit (PE)

abx290009-100test 100 test
EUR 495
  • Shipped within 2-3 weeks.

Annexin V / PI Apoptosis Detection Kit (Biotin)

abx290011-100test 100 test
EUR 620
  • Shipped within 2-3 weeks.

Annexin V Apoptosis Detection Kit (Dylight 634)

abx290016-200test 200 test
EUR 537
  • Shipped within 3-5 working days.

Annexin V Apoptosis Detection Kit (CF-Blue)

abx290018-200test 200 test
EUR 620
  • Shipped within 3-5 working days.

Aposcreen Annexin V Apoptosis Kit : FITC (OKSB00007)

OKSB00007 100 Tests
EUR 513
Description: Description of target: ;Species reactivity: ;Application: FC, ICC;Assay info: ;Sensitivity:

Aposcreen Annexin V Apoptosis Kit : PE (OKSB00008)

OKSB00008 100 Tests
EUR 513
Description: Description of target: ;Species reactivity: ;Application: FC;Assay info: The ApoScreen Annexin V Apoptosis Kit employs a fluorescein- labeled annexin V (annexin V- FITC) in concert withpropidium iodide (PI) to evaluate subpopulations of cells undergoing apoptosis. During the early stages of apoptosis,cells begin to display PS on the outer cell membrane where it is readily detectable by staining the cells with annexin VFITC.As the plasma membrane becomes increasingly permeable during the later stages of apoptosis, PI can readilymove across the cell membrane and bind to cellular DNA, providing a means for identifying those cells that have lostmembrane integrity through mechanisms including necrosis. When cells are double stained with annexin V- FITC and PI,three different cell populations may be observed (Figure 2): (i) live cells that do not stain with either annexin V- FITC or PI;(ii) necrotic cells that stain with both reagents; and (iii) apoptotic cells that stain with annexin V- FITC only. Analysis canbe performed on any flow cytometer equipped with a single laser using excitation at 488 nm. Alternatively, the cells canbe examined with a fluorescence microscope equipped with filters for discrimination of FITC and rhodamine/PI.;Sensitivity:

Annexin V Apoptosis Detection Kit : APC (OKTA00002)

OKTA00002 KIT
EUR 330
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Annexin V Apoptosis Detection Kit : FITC (OKTA00003)

OKTA00003 KIT
EUR 317
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Annexin V Apoptosis Detection Kit : PE (OKTA00005)

OKTA00005 KIT
EUR 304
Description: Description of target: ;Species reactivity: ;Application: FC;Assay info: ;Sensitivity:

Annexin V-PE-Cy5 Apoptosis Detection Kit

K2201-100 100 assays
EUR 557

Annexin V-PE-Cy5 Apoptosis Detection Kit

K2201-25 25 assays
EUR 293

Annexin V-FITC/PI Apoptosis Detection Kit

A211-01 50 rxn
EUR 216

Annexin V-FITC/PI Apoptosis Detection Kit

A211-02 100 rxn
EUR 295

Annexin V-PE-Cy5 Apoptosis Detection Kit

K129-100
EUR 490

Annexin V-PE-Cy5 Apoptosis Detection Kit

K129-25
EUR 251

Annexin V Apoptosis Detection Kit (PE) (CY5)

55R-1289 25 assays
EUR 347
Description: Annexin V Apoptosis Kit for detection of apoptosis in the research laboratory

Annexin V Apoptosis Detection Kit Plus (FITC)

55R-1302 25 assays
EUR 296
Description: Annexin V Apoptosis Kit for detection of apoptosis in the research laboratory

Annexin V Apoptosis Detection Kit Plus (CY3)

55R-1303 25 assays
EUR 296
Description: Annexin V Apoptosis Kit for detection of apoptosis in the research laboratory

Annexin V Apoptosis Detection Kit Plus (PE)

55R-1304 25 assays
EUR 303
Description: Annexin V Apoptosis Kit for detection of apoptosis in the research laboratory

Annexin V (FITC) / 7-AAD Apoptosis Detection Kit

abx090603-100tests 100 tests
EUR 495
  • Shipped within 5-10 working days.

Annexin V (APC) / PI Apoptosis Detection Kit (APC)

abx090605-100tests 100 tests
EUR 648
  • Shipped within 5-10 working days.

Annexin V-FITC / PI Cell Apoptosis Detection Kit

20-abx098246
  • EUR 244.00
  • EUR 314.00
  • 25 rxns
  • 50 rxns
  • Shipped within 5-10 working days.

Annexin V / PI Apoptosis Detection Kit (Dylight 634)

abx290010-100test 100 test
EUR 537
  • Shipped within 3-5 working days.

Annexin V / PI Apoptosis Detection Kit (CF-Blue)

abx290012-100test 100 test
EUR 565
  • Shipped within 3-5 working days.

Annexin V / 7AAD Apoptosis Detection Kit (CF-Blue)

abx290013-100test 100 test
EUR 565
  • Shipped within 3-5 working days.

Annexin V-PE/7-AAD Apoptosis Detection Kit

A213-01 50 rxn
EUR 239

Annexin V-PE/7-AAD Apoptosis Detection Kit

A213-02 100 rxn
EUR 308

Dual Apoptosis Assay with NucView 488 Caspase-3 Substrate and CF594 Annexin V (50 assays)

30067 1KIT
EUR 429
Description: Minimum order quantity: 1 unit of 1KIT

Dual Apoptosis Assay with NucView 488 Caspase-3 Substrate and CF640R-Annexin V (50 assays)

30073 1KIT
EUR 429
Description: Minimum order quantity: 1 unit of 1KIT

Lastly, the gene expression evaluation by (RT-qPCR) methodology was carried out by analyzing the expression of the gene of curiosity for the quantification of mRNA ranges. Results after cells handled with IC50 have been revealed by upregulating anti-apoptotic genes/downregulated of pro-apoptotic BCL-2 gene expressions have been triggered the handled cells into CASPASE-3, intrinsic and extrinsic pathways. These findings recommend that the methanol extracts of three elements of A. altilis fruit have potential anticancer exercise towards MCF-7 cells primarily the pulp a part of the fruit.